hiscript ii reverse transcriptase kit (Vazyme Biotech Co)
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hiscript ii reverse transcriptase kit
Hiscript Ii Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/HiScript+II+Reverse+Transcriptase/pm42873856-614-10-15
Average 97 stars, based on 1513 article reviews
Hiscript Ii Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcriptase+kit/HiScript+II+Reverse+Transcriptase/pm42873856-614-10-15
Average 97 stars, based on 1513 article reviews
hiscript ii reverse transcriptase kit - by Bioz Stars,
2026-11
97/100 stars
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RNA Extraction:Article Title: Novel Mycoplasma bovis membrane lipoproteins induce the inflammatory response of host epithelial cells and macrophage Article Snippet: MAC-T, BoMac and EBL cells, each at a density of 1×10 6 cells per well, were seeded into six-well plates and after allowing the cells to adhere overnight, recombinant protein was added to stimulate the cells as required by the experimental protocol. .. This was followed by total RNA extraction using Trizol reagent (#15596018CN, Thermo Fisher Scientific, USA), after which a corresponding cDNA was synthesized through reverse transcription with a Synthesized:Article Title: Novel Mycoplasma bovis membrane lipoproteins induce the inflammatory response of host epithelial cells and macrophage Article Snippet: MAC-T, BoMac and EBL cells, each at a density of 1×10 6 cells per well, were seeded into six-well plates and after allowing the cells to adhere overnight, recombinant protein was added to stimulate the cells as required by the experimental protocol. .. This was followed by total RNA extraction using Trizol reagent (#15596018CN, Thermo Fisher Scientific, USA), after which a corresponding cDNA was synthesized through reverse transcription with a Article Title: Effects of Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri on oral microbial balance and host cell functions: Implications for the prevention and management of oral diseases. Article Snippet: Objectives: This study aimed to evaluate the effects of three probiotic samples—Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri—on oral pathogens, commensal bacteria, and host oral cells, thereby exploring their potential roles in maintaining microbial balance and promoting host defense.. Design: Probiotic culture supernatants (postbiotic fractions) were tested against oral pathogens (S. mutans, P. gingivalis, F. nucleatum, A. actinomycetemcomitans) and commensal species (S. sanguinis, V. parvula) using optical density assays.. Primary human gingival fibroblasts and oral keratinocytes were co-cultured with probiotic preparations to assess cell viability, inflammatory cytokine secretion, and barrier gene expression (FLG, LOR). Reverse Transcription:Article Title: Novel Mycoplasma bovis membrane lipoproteins induce the inflammatory response of host epithelial cells and macrophage Article Snippet: MAC-T, BoMac and EBL cells, each at a density of 1×10 6 cells per well, were seeded into six-well plates and after allowing the cells to adhere overnight, recombinant protein was added to stimulate the cells as required by the experimental protocol. .. This was followed by total RNA extraction using Trizol reagent (#15596018CN, Thermo Fisher Scientific, USA), after which a corresponding cDNA was synthesized through reverse transcription with a Article Title: Long-term exercise enhances meningeal lymphatic vessel plasticity and drainage in a mouse model of Alzheimer's disease Article Snippet: TSP-1 and EAF2 levels in the CSF and hippocampus of mice, as well as in the pellet and supernatant of primary astrocytes, were determined with ELISA commercial kits (Jingmei Co., Ltd. Cat# JM-04106H1, JM-13432M2) following the manufacturer’s instructions. .. Total RNA was extracted using Trizol reagent (TaKaRa, Kusatsu, Japan), and cDNA was generated using a Article Title: Dietary 5-hydroxytryptophan supplementation improves growth and metabolism of large yellow croaker (Larimichthys crocea) in association with microbiota-gut-brain axis-related changes Article Snippet: .. First-strand cDNA synthesis of RNA was conducted using a Article Title: Effects of Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri on oral microbial balance and host cell functions: Implications for the prevention and management of oral diseases. Article Snippet: Objectives: This study aimed to evaluate the effects of three probiotic samples—Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri—on oral pathogens, commensal bacteria, and host oral cells, thereby exploring their potential roles in maintaining microbial balance and promoting host defense.. Design: Probiotic culture supernatants (postbiotic fractions) were tested against oral pathogens (S. mutans, P. gingivalis, F. nucleatum, A. actinomycetemcomitans) and commensal species (S. sanguinis, V. parvula) using optical density assays.. Primary human gingival fibroblasts and oral keratinocytes were co-cultured with probiotic preparations to assess cell viability, inflammatory cytokine secretion, and barrier gene expression (FLG, LOR). Article Title: Therapeutic Potential of Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles in Atherosclerotic Stenosis and Endothelial Dysfunction. Article Snippet: Atherosclerotic stenosis is a progressive vascular disease characterized by the accumulation of lipids, immune cells, and fibrous tissue within arterial walls, leading to vascular narrowing [1, 2].. The clinical manifestations of atherosclerotic stenosis depend on the location and severity of the stenosis and the involved organ [3, 4].. Diabetes mellitus, a common metabolic disorder, exacerbates the progression of atherosclerosis, posing a considerable challenge to effective treatment strategies [5]. Article Title: Proteasome inhibition induces DNA methylation alteration by attenuating the synthesis of DNA methyltransferase 1 and 3B in colorectal cancer. Article Snippet: Total RNA was isolated and genomic DNA was removed by the RNA extraction kit (Vazyme, Cat# RC112-01) following the manufacturer’s instructions. .. Random-primed reverse transcription of RNA (1 μg) was performed using Generated:Article Title: Long-term exercise enhances meningeal lymphatic vessel plasticity and drainage in a mouse model of Alzheimer's disease Article Snippet: TSP-1 and EAF2 levels in the CSF and hippocampus of mice, as well as in the pellet and supernatant of primary astrocytes, were determined with ELISA commercial kits (Jingmei Co., Ltd. Cat# JM-04106H1, JM-13432M2) following the manufacturer’s instructions. .. Total RNA was extracted using Trizol reagent (TaKaRa, Kusatsu, Japan), and cDNA was generated using a Quantitative RT-PCR:Article Title: Long-term exercise enhances meningeal lymphatic vessel plasticity and drainage in a mouse model of Alzheimer's disease Article Snippet: TSP-1 and EAF2 levels in the CSF and hippocampus of mice, as well as in the pellet and supernatant of primary astrocytes, were determined with ELISA commercial kits (Jingmei Co., Ltd. Cat# JM-04106H1, JM-13432M2) following the manufacturer’s instructions. .. Total RNA was extracted using Trizol reagent (TaKaRa, Kusatsu, Japan), and cDNA was generated using a Real-time Polymerase Chain Reaction:Article Title: Long-term exercise enhances meningeal lymphatic vessel plasticity and drainage in a mouse model of Alzheimer's disease Article Snippet: TSP-1 and EAF2 levels in the CSF and hippocampus of mice, as well as in the pellet and supernatant of primary astrocytes, were determined with ELISA commercial kits (Jingmei Co., Ltd. Cat# JM-04106H1, JM-13432M2) following the manufacturer’s instructions. .. Total RNA was extracted using Trizol reagent (TaKaRa, Kusatsu, Japan), and cDNA was generated using a Expressing:Article Title: Long-term exercise enhances meningeal lymphatic vessel plasticity and drainage in a mouse model of Alzheimer's disease Article Snippet: TSP-1 and EAF2 levels in the CSF and hippocampus of mice, as well as in the pellet and supernatant of primary astrocytes, were determined with ELISA commercial kits (Jingmei Co., Ltd. Cat# JM-04106H1, JM-13432M2) following the manufacturer’s instructions. .. Total RNA was extracted using Trizol reagent (TaKaRa, Kusatsu, Japan), and cDNA was generated using a cDNA Synthesis:Article Title: Dietary 5-hydroxytryptophan supplementation improves growth and metabolism of large yellow croaker (Larimichthys crocea) in association with microbiota-gut-brain axis-related changes Article Snippet: .. First-strand cDNA synthesis of RNA was conducted using a Article Title: Therapeutic Potential of Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles in Atherosclerotic Stenosis and Endothelial Dysfunction. Article Snippet: Atherosclerotic stenosis is a progressive vascular disease characterized by the accumulation of lipids, immune cells, and fibrous tissue within arterial walls, leading to vascular narrowing [1, 2].. The clinical manifestations of atherosclerotic stenosis depend on the location and severity of the stenosis and the involved organ [3, 4].. Diabetes mellitus, a common metabolic disorder, exacerbates the progression of atherosclerosis, posing a considerable challenge to effective treatment strategies [5]. Purification:Article Title: Effects of Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri on oral microbial balance and host cell functions: Implications for the prevention and management of oral diseases. Article Snippet: Objectives: This study aimed to evaluate the effects of three probiotic samples—Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri—on oral pathogens, commensal bacteria, and host oral cells, thereby exploring their potential roles in maintaining microbial balance and promoting host defense.. Design: Probiotic culture supernatants (postbiotic fractions) were tested against oral pathogens (S. mutans, P. gingivalis, F. nucleatum, A. actinomycetemcomitans) and commensal species (S. sanguinis, V. parvula) using optical density assays.. Primary human gingival fibroblasts and oral keratinocytes were co-cultured with probiotic preparations to assess cell viability, inflammatory cytokine secretion, and barrier gene expression (FLG, LOR). Amplification:Article Title: Barley husk degradation driven by Fusarium graminearum xylanase triggers premature yeast flocculation factor generation Article Snippet: 1 Department of Biological and Food Engineering, Jilin University of Chemical Technology, Jilin 132022, China 2 Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China 3 JSFEC Malting Co., Ltd, Sheyang 224300, China 4 National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China Abstract Premature yeast flocculation (PYF) is a detrimental phenomenon in beer brewing that causes yeast to clump and settle too early, severely impacting fermentation efficiency and beer flavor.. Although it is known that this issue is related to barley infection with fungi such as Fusarium graminearum, the exact biochemical triggering factors are still unclear, preventing the development of targeted prevention protocols.. We assume that a specific type of xylanase secreted by F. graminearum can break down barley husk components and is a key driving factor for PYF. Random Primed:Article Title: Proteasome inhibition induces DNA methylation alteration by attenuating the synthesis of DNA methyltransferase 1 and 3B in colorectal cancer. Article Snippet: Total RNA was isolated and genomic DNA was removed by the RNA extraction kit (Vazyme, Cat# RC112-01) following the manufacturer’s instructions. .. Random-primed reverse transcription of RNA (1 μg) was performed using |